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microvessel counting under high-power magnification microscopy  (KEYENCE)
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KEYENCE microvessel counting under high-power magnification microscopy
Immunohistopathological analysis of CD34 staining. ( A ) <t>Microvessel</t> density (MVD) and diameter analyzed using CD34 staining are shown. Large CD34(+) vessels are observed in the tumors with DLM. Few CD34(+) vessels are identified in DLM (scale bar= 100 μm). ( B ) No significant difference is observed in the MVD in the tumors between DLM group and non-DLM groups ( p = 0.19). Vessel diameters are significantly larger in DLM group than that in non-DLM group ( p < 0.01). ( C ) The correlation between tumor volume and MVD are shown. The larger-sized VSs demonstrate higher MVD in the tumor with DLM ( p < 0.05, r = 0.89).
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Immunohistopathological analysis of CD34 staining. ( A ) Microvessel density (MVD) and diameter analyzed using CD34 staining are shown. Large CD34(+) vessels are observed in the tumors with DLM. Few CD34(+) vessels are identified in DLM (scale bar= 100 μm). ( B ) No significant difference is observed in the MVD in the tumors between DLM group and non-DLM groups ( p = 0.19). Vessel diameters are significantly larger in DLM group than that in non-DLM group ( p < 0.01). ( C ) The correlation between tumor volume and MVD are shown. The larger-sized VSs demonstrate higher MVD in the tumor with DLM ( p < 0.05, r = 0.89).

Journal: Brain Sciences

Article Title: Histopathological Investigation of Dura-like Membrane in Vestibular Schwannomas

doi: 10.3390/brainsci11121649

Figure Lengend Snippet: Immunohistopathological analysis of CD34 staining. ( A ) Microvessel density (MVD) and diameter analyzed using CD34 staining are shown. Large CD34(+) vessels are observed in the tumors with DLM. Few CD34(+) vessels are identified in DLM (scale bar= 100 μm). ( B ) No significant difference is observed in the MVD in the tumors between DLM group and non-DLM groups ( p = 0.19). Vessel diameters are significantly larger in DLM group than that in non-DLM group ( p < 0.01). ( C ) The correlation between tumor volume and MVD are shown. The larger-sized VSs demonstrate higher MVD in the tumor with DLM ( p < 0.05, r = 0.89).

Article Snippet: To assess microvessel density (MVD) and vessel diameter, tissue sections were screened using CD34 immunohistochemistry in low-power fields and the three most vascularized regions (hot spots) were selected for automatic microvessel counting under high-power magnification microscopy (0.95 mm 2 ) (Biorevo BZ-9000, KEYENCE, Osaka, Japan), as previously described [ ].

Techniques: Staining